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MYLK 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00004638-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-MYLK full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 109.23
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-MYLK antibody (H00004638-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00004638-T01
    MYLK transfected lysate.
    Western Blot
    QC Testing of H00004638-T01
    Lane 1: MYLK transfected lysate ( 109.23 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 4638
  • Gene Name:
  • MYLK
  • Gene Alias:
  • DKFZp686I10125,FLJ12216,KRP,MLCK,MLCK1,MLCK108,MLCK210,MSTP083,MYLK1,smMLCK
  • Gene Description:
  • myosin light chain kinase
  • Gene Summary:
  • This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000180642,OTTHUMP00000180643,myosin, light polypeptide kinase,smooth muscle myosin light chain kinase
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