MYCN (Human) Recombinant Protein (P01)
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More Files
- More Functions
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Specification
Product Description
Human MYCN full-length ORF ( NP_005369.2, 1 a.a. - 464 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MPSCSTSTMPGMICKNPDLEFDSLQPCFYPDEDDFYFGGPDSTPPGEDIWKKFELLPTPPLSPSRGFAEHSSEPPSWVTEMLLENELWGSPAEEDAFGLGGLGGLTPNPVILQDCMWSGFSAREKLERAVSEKLQHGRGPPTAGSTAQSPGAGAASPAGRGHGGAAGAGRAGAALPAELAHPAAECVDPAVVFPFPVNKREPAPVPAAPASAPAAGPAVASGAGIAAPAGAPGVAPPRPGGRQTSGGDHKALSTSGEDTLSDSDDEDDEEEDEEEEIDVVTVEKRRSSSNTKAVTTFTITVRPKNAALGPGRAQSSELILKRCLPIHQQHNYAAPSPYVESEDAPPQKKIKSEASPRPLKSVIPPKAKSLSPRNSDSEDSERRRNHNILERQRRNDLRSSFLTLRDHVPELVKNEKAAKVVILKKATEYVHSLQAEEHQLLLEKEKLQARQQQLLKKIEHARTC
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
76
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — MYCN
Entrez GeneID
4613GeneBank Accession#
NM_005378.4Protein Accession#
NP_005369.2Gene Name
MYCN
Gene Alias
MODED, N-myc, NMYC, ODED, bHLHe37
Gene Description
v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)
Gene Ontology
HyperlinkGene Summary
This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. [provided by RefSeq
Other Designations
N-myc proto-oncogene protein|neuroblastoma MYC oncogene|neuroblastoma-derived v-myc avian myelocytomatosis viral related oncogene|oncogene NMYC|pp65/67|v-myc avian myelocytomatosis viral related oncogene, neuroblastoma derived|v-myc myelocytomatosis viral
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Interactome
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Disease
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Publication Reference
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Novel pharmacodynamic biomarkers for MYCN protein and PI3K/AKT/mTOR pathway signaling in children with neuroblastoma.
Smith JR, Moreno L, Heaton SP, Chesler L, Pearson ADJ, Garrett MD.
Molecular Oncology 2016 Apr; 10(4):538.
Application：WB, Human, Neuroblastoma cells.
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NCYM promotes calpain-mediated Myc-nick production in human MYCN-amplified neuroblastoma cells.
Shoji W, Suenaga Y, Kaneko Y, Islam SM, Alagu J, Yokoi S, Nio M, Nakagawara A.
Biochemical and Biophysical Research Communications 2015 Jun; 461(3):501.
Application：WB, Human, Purified Protein.
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A matrix approach to guide IHC-based tissue biomarker development in oncology drug discovery.
Smith NR, Womack C.
The Journal of Pathology 2014 Jan; 232(2):190.
Application：WB, Recombinant protein.
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NCYM, a Cis-Antisense Gene of MYCN, Encodes a De Novo Evolved Protein That Inhibits GSK3β Resulting in the Stabilization of MYCN in Human Neuroblastomas.
Suenaga Y, Islam SM, Alagu J, Kaneko Y, Kato M, Tanaka Y, Kawana H, Hossain S, Matsumoto D, Yamamoto M, Shoji W, Itami M, Shibata T, Nakamura Y, Ohira M, Haraguchi S, Takatori A, Nakagawara A.
PLoS Genetics 2014 Jan; 10(1):e1003996.
Application：Pull-Down, KA, Human, CHP134 cells.
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Novel pharmacodynamic biomarkers for MYCN protein and PI3K/AKT/mTOR pathway signaling in children with neuroblastoma.
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