MYCN monoclonal antibody (M01), clone 3H4
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant MYCN.
Immunogen
MYCN (NP_005369, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MPSCSTSTMPGMICKNPDLEFDSLQPCFYPDEDDFYFGGPDSTPPGEDIWKKFELLPTPPLSPSRGFAEHSSEPPSWVTEMLLENELWGSPAEEDAFGLG
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
MYCN monoclonal antibody (M01), clone 3H4 Western Blot analysis of MYCN expression in HepG2 ( Cat # L019V1 ).Western Blot (Transfected lysate)
Western Blot analysis of MYCN expression in transfected 293T cell line by MYCN monoclonal antibody (M01), clone 3H4.
Lane 1: MYCN transfected lysate(49.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MYCN is approximately 0.03ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of MYCN over-expressed 293 cell line, cotransfected with MYCN Validated Chimera RNAi ( Cat # H00004613-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MYCN monoclonal antibody (M01), clone 3H4 (Cat # H00004613-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — MYCN
Entrez GeneID
4613GeneBank Accession#
NM_005378Protein Accession#
NP_005369Gene Name
MYCN
Gene Alias
MODED, N-myc, NMYC, ODED, bHLHe37
Gene Description
v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)
Gene Ontology
HyperlinkGene Summary
This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. [provided by RefSeq
Other Designations
N-myc proto-oncogene protein|neuroblastoma MYC oncogene|neuroblastoma-derived v-myc avian myelocytomatosis viral related oncogene|oncogene NMYC|pp65/67|v-myc avian myelocytomatosis viral related oncogene, neuroblastoma derived|v-myc myelocytomatosis viral
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Interactome
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Disease
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