This antibody pair set comes with matched antibody pair to detect and quantify protein level of human MUC1.
Quality Control Testing:
Standard curve using recombinant protein ( H00004582-Q01 ) as an analyte.
Sandwich ELISA detection sensitivity ranging from 3 ng/ml to 100 ng/ml.
Antibody pair set content: 1. Capture antibody: mouse monoclonal anti-MUC1, IgG2b Kappa (100 ug) 2. Detection antibody: biotinylated mouse monoclonal anti-MUC1, IgG2a Kappa (50 ug) *Reagents are sufficient for at least 3-5 x 96 well plates using recommended protocols.
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
Standard curve using MUC1 293T overexpression lysate (non-denatured) as an analyte. Sandwich ELISA detection sensitivity ranging from approximately 729x to 3x dilution of the MUC1 293T overexpression lysate (non-denatured).
This gene is a member of the mucin family and encodes a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length nature of only some has been determined. [provided by RefSeq