MTHFD1 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MTHFD1 full-length
Host
Human
Theoretical MW (kDa)
102.96
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MTHFD1 antibody (H00004522-B01) by Western Blots.
SDS-PAGE Gel
MTHFD1 transfected lysate.
Western Blot
Lane 1: MTHFD1 transfected lysate ( 102.96 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MTHFD1
Entrez GeneID
4522GeneBank Accession#
BC050420.1Protein Accession#
AAH50420.1Gene Name
MTHFD1
Gene Alias
MTHFC, MTHFD
Gene Description
methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. [provided by RefSeq
Other Designations
5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate cyclohydrolase, 10-formyltetrahydrofolate synthetase|C1-THF synthase|NADP-dependent cyclohydrolase/formyltetrahydrofolate synthetase|cytoplasmic C-1-tetrahydrofolate synthase|met
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Interactome
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Pathway
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Disease
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