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Last updated: 2022/8/14

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MTHFD1 293T Cell Transient Overexpression Lysate(Denatured) 

  • Catalog # : H00004522-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-MTHFD1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 102.96
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-MTHFD1 antibody (H00004522-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00004522-T01
    MTHFD1 transfected lysate.
    Western Blot
    QC Testing of H00004522-T01
    Lane 1: MTHFD1 transfected lysate ( 102.96 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 4522
  • Gene Name:
  • MTHFD1
  • Gene Alias:
  • MTHFC,MTHFD
  • Gene Description:
  • methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
  • Gene Summary:
  • This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. [provided by RefSeq
  • Other Designations:
  • 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate cyclohydrolase, 10-formyltetrahydrofolate synthetase,C1-THF synthase,NADP-dependent cyclohydrolase/formyltetrahydrofolate synthetase,cytoplasmic C-1-tetrahydrofolate synthase,met
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