MTHFD1 (Human) IP-WB Antibody Pair
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Specification
Product Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity
Human
Quality Control Testing
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of MTHFD1 transfected lysate using rabbit polyclonal anti-MTHFD1 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-MTHFD1.
Supplied Product
Antibody pair set content:
1. Antibody pair for IP: rabbit polyclonal anti-MTHFD1 (300 ul)
2. Antibody pair for WB: mouse purified polyclonal anti-MTHFD1 (50 ug)
Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
Immunoprecipitation-Western Blot
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Gene Info — MTHFD1
Entrez GeneID
4522Gene Name
MTHFD1
Gene Alias
MTHFC, MTHFD
Gene Description
methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. [provided by RefSeq
Other Designations
5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate cyclohydrolase, 10-formyltetrahydrofolate synthetase|C1-THF synthase|NADP-dependent cyclohydrolase/formyltetrahydrofolate synthetase|cytoplasmic C-1-tetrahydrofolate synthase|met
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Disease
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