MTHFD1 purified MaxPab mouse polyclonal antibody (B01P)
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More Files
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Western Blot (Tissue lysate)
MTHFD1 MaxPab polyclonal antibody. Western Blot analysis of MTHFD1 expression in human placenta.
Western Blot (Cell lysate)
MTHFD1 MaxPab polyclonal antibody. Western Blot analysis of MTHFD1 expression in HeLa.
Western Blot (Transfected lysate)
Western Blot analysis of MTHFD1 expression in transfected 293T cell line (H00004522-T01) by MTHFD1 MaxPab polyclonal antibody.
Lane1:MTHFD1 transfected lysate(102.85 KDa).
Lane2:Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to MTHFD1 on HeLa cell. [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human MTHFD1 protein.
Immunogen
MTHFD1 (AAH50420.1, 1 a.a. ~ 935 a.a) full-length human protein.
Sequence
MAPAEILNGKEISAQIRARLKNQVTQLKEQVPGFTPRLAILQVGNRDDSNLYINVKLKAAEEIGIKATHIKLPRTTTESEVMKYITSLNEDSTVHGFLVQLPLDSENSINTEEVINAIAPEKDVDGLTSINAGRLARGDLNDCFIPCTPKGCLELIKETGVPIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTAHLDEEVNKGDILVVATGQPEMVKGEWIKPGAIVIDCGINYVPDDKKPNGRKVVGDVAYDEAKERASFITPVPGGVGPMTVAMLMQSTVESAKRFLEKFKPGKWMIQYNNLNLKTPVPSDIDISRSCKPKPIGKLAREIGLLSEEVELYGETKAKVLLSALERLKHRPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLYQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARIFHELTQTDKALFNRLVPSVNGVRRFSDIQIRRLKRLGIEKTDPTTLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQAPTEKGHTRTAQFDISVASEIMAVLALTTSLEDMRERLGKMVVASSKKGEPVSAEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLCPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYIQENLELVEKGFSNLKKQIENARMFGIPVVVAVNAFKTDTESELDFISRLSREHGAFDAVKCTHWAEGGKGALALAQAVQRAAQAPSSFQLLYDLKLPVEDKIRIIAQKIYGADDIELLPEAQHKAEVYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFILPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDLDPETEQVNGLF
Host
Mouse
Reactivity
Human
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
MTHFD1 MaxPab polyclonal antibody. Western Blot analysis of MTHFD1 expression in human placenta.Western Blot (Cell lysate)
MTHFD1 MaxPab polyclonal antibody. Western Blot analysis of MTHFD1 expression in HeLa.Western Blot (Transfected lysate)
Western Blot analysis of MTHFD1 expression in transfected 293T cell line (H00004522-T01) by MTHFD1 MaxPab polyclonal antibody.
Lane1:MTHFD1 transfected lysate(102.85 KDa).
Lane2:Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to MTHFD1 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — MTHFD1
Entrez GeneID
4522GeneBank Accession#
BC050420.1Protein Accession#
AAH50420.1Gene Name
MTHFD1
Gene Alias
MTHFC, MTHFD
Gene Description
methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. [provided by RefSeq
Other Designations
5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate cyclohydrolase, 10-formyltetrahydrofolate synthetase|C1-THF synthase|NADP-dependent cyclohydrolase/formyltetrahydrofolate synthetase|cytoplasmic C-1-tetrahydrofolate synthase|met
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Interactome
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Pathway
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Disease
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