MSI1 monoclonal antibody (M04), clone 3F2
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More Files
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Specifications
Product Description
Mouse monoclonal antibody raised against a full length recombinant MSI1.
Immunogen
MSI1 (NP_002433, 1 a.a. ~ 87 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
METDAPQPGL ASPDSPHDPC KMFIGGLSWQ TTQEGLREYF GQFGE VKECL VMRDPLTKRS RGFGFVTFMD QAGVDKVLAQ SRHELDS
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.57 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
MSI1 monoclonal antibody (M04), clone 3F2 Western Blot analysis of MSI1 expression in IMR-32 ( Cat # L008V1 ).Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to MSI1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MSI1 is approximately 0.3ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to MSI1 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — MSI1
Entrez GeneID
4440GeneBank Accession#
NM_002442Protein Accession#
NP_002433Gene Name
MSI1
Gene Alias
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Gene Description
musashi homolog 1 (Drosophila)
Omim ID
603328Gene Ontology
HyperlinkGene Summary
This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13. [provided by RefSeq
Other Designations
musashi 1
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Interactomes
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Publication Reference
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Thyroid hormone-induced expression of Foxl1 in subepithelial fibroblasts correlates with adult stem cell development during Xenopus intestinal remodeling.
Takashi Hasebe, Kenta Fujimoto, Atsuko Ishizuya-Oka.
Scientific Reports 2020 Nov; 10(1):20715.
Application:IF, IHC-P, WB-Tr, Frog, Frog small intestine, In vitro-translated proteins.
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Drug-resistant colon cancer cells produce high carcinoembryonic antigen and might not be cancer-initiating cells.
Lee HC, Ling QD, Yu WC, Hung CM, Kao TC, Huang YW, Higuchi A.
Drug Design, Development and Therapy 2013 Jun; 7:491.
Application:IS, Human, LoVo cells.
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Thyroid hormone-induced expression of Foxl1 in subepithelial fibroblasts correlates with adult stem cell development during Xenopus intestinal remodeling.
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