MSH5 monoclonal antibody (M08), clone 1C11
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant MSH5.
Immunogen
MSH5 (AAH02498, 736 a.a. ~ 835 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
GNDLVFFYQVCEGVAKASHASHTAAQAGLPDKLVARGKEVSDLIRSGKPIKPVKDLLKKNQMENCQTLVDKFMKLDLEDPNLDLNVFMSQEVLPAATSIL
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (79); Rat (78)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MSH5 is approximately 0.1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to MSH5 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — MSH5
Entrez GeneID
4439GeneBank Accession#
BC002498Protein Accession#
AAH02498Gene Name
MSH5
Gene Alias
DKFZp434C1615, G7, MGC2939, MutSH5, NG23
Gene Description
mutS homolog 5 (E. coli)
Omim ID
603382Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the mutS family of proteins that are involved in DNA mismatch repair or meiotic recombination processes. This protein is similar to a Saccharomyces cerevisiae protein that participates in meiotic segregation fidelity and crossing-over. This protein forms heterooligomers with another member of this family, mutS homolog 4. Alternative splicing results in four transcript variants encoding three different isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000029187|OTTHUMP00000029188|mutS homolog 5
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Interactome
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Disease
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Publication Reference
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Spermatogenesis-specific association of SMCY and MSH5.
Akimoto C, Kitagawa H, Matsumoto T, Kato S.
Genes to Cells : Devoted to Molecular & Cellular Mechanisms 2008 May; 13(6):623.
Application:IF, IHC-P, WB, Human, Mouse, Mouse testis, NEC8 cells.
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Spermatogenesis-specific association of SMCY and MSH5.
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