MPST 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MPST transfected lysate.
Western Blot
Lane 1: MPST transfected lysate ( 32.78 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MPST full-length
Host
Human
Theoretical MW (kDa)
32.78
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MPST antibody (H00004357-B01) by Western Blots.
SDS-PAGE Gel
MPST transfected lysate.
Western Blot
Lane 1: MPST transfected lysate ( 32.78 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MPST
Entrez GeneID
4357GeneBank Accession#
NM_021126.4Protein Accession#
NP_066949.1Gene Name
MPST
Gene Alias
MGC24539, MST, TST2
Gene Description
mercaptopyruvate sulfurtransferase
Omim ID
602496Gene Ontology
HyperlinkGene Summary
This protein encoded by this gene catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein (mercaptopyruvate sulfurtransferase, MPST), which appears to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese, TST, GeneID:7263), which is a mitochondrial protein. Deficiency in MPST activity has been implicated in a rare inheritable disorder known as mercaptolactate-cysteine disulfiduria (MCDU). Alternatively spliced transcript variants encoding same or different isoforms have been identified for this gene. [provided by RefSeq
Other Designations
3-mercaptopyruvate sulfurtransferase|OTTHUMP00000028670|human liver rhodanese
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