MPP3 monoclonal antibody (M01), clone 4D7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of MPP3 expression in transfected 293T cell line by MPP3 monoclonal antibody (M01), clone 4D7.
Lane 1: MPP3 transfected lysate(66.2 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MPP3 is approximately 0.03ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of MPP3 over-expressed 293 cell line, cotransfected with MPP3 Validated Chimera RNAi ( Cat # H00004356-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MPP3 monoclonal antibody (M01), clone 4D7 (Cat # H00004356-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (90.09 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant MPP3.
Immunogen
MPP3 (AAH56865, 1 a.a. ~ 585 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MPVLSEDSGLHETLALLTSQLRPDSNHKEEMGFLRDVFSEKSLSYLMKIHEKLRYYERQSPTPVLHSAVALAEDVMEELQAASVHSDERELLQLLSTPHLRAVLMVHDTVAQKNFDPVLPPLPDNIDEDFDEESVKIVRLVKNKEPLGATIRRDEHSGAVVVARIMRGGAADRSGLVHVGDELREVNGIAVLHKRPDEISQILAQSQGSITLKIIPATQEEDRLKESKVFMRALFHYNPREDRAIPCQEAGLPFQRRQVLEVVSQDDPTWWQAKRVGDTNLRAGLIPSKGFQERRLSYRRAAGTLPSPQSLRKPPYDQPCDKETCDCEGYLKGHYVAGLRRSFRLGCRERLGGSQEGKMSSGAESPELLTYEEVARYQHQPGERPRLVVLIGSLGARLHELKQKVVAENPQHFGVAVPHTTRPRKSHEKEGVEYHFVSKQAFEADLHHNKFLEHGEYKENLYGTSLEAIQAVMAKNKVCLVDVEPEALKQLRTSEFKPYIIFVKPAIQEKRKTPPMSPACEDTAAPFDEQQQEMAASAAFIDRHYGHLVDAVLVKEDLQGAYSQLKVVLEKLSKDTHWVPVSWVR
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (90.09 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of MPP3 expression in transfected 293T cell line by MPP3 monoclonal antibody (M01), clone 4D7.
Lane 1: MPP3 transfected lysate(66.2 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MPP3 is approximately 0.03ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of MPP3 over-expressed 293 cell line, cotransfected with MPP3 Validated Chimera RNAi ( Cat # H00004356-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MPP3 monoclonal antibody (M01), clone 4D7 (Cat # H00004356-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — MPP3
Entrez GeneID
4356GeneBank Accession#
BC056865Protein Accession#
AAH56865Gene Name
MPP3
Gene Alias
DLG3
Gene Description
membrane protein, palmitoylated 3 (MAGUK p55 subfamily member 3)
Omim ID
601114Gene Ontology
HyperlinkGene Summary
This gene product is a member of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. This protein contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction. Alternatively spliced transcript variants have been identified. One transcript variant is experimentally supported, but it doesn't encode a protein. [provided by RefSeq
Other Designations
MAGUK p55 subfamily member 3|OTTHUMP00000165325|discs, large homolog 3|membrane protein palmitoylated 3|palmitoylated membrane protein 3
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Interactome
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