MPP1 monoclonal antibody (M02), clone 1C3-1D11
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More Files
- More Functions
Western Blot (Cell lysate)
MPP1 monoclonal antibody (M02), clone 1E11-1G11 Western Blot analysis of MPP1 expression in K-562 ( Cat # L009V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of MPP1 expression in transfected 293T cell line by MPP1 monoclonal antibody (M02), clone 1C3-1D11.
Lane 1: MPP1 transfected lysate(52.3 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MPP1 is approximately 3ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of MPP1 over-expressed 293 cell line, cotransfected with MPP1 Validated Chimera RNAi ( Cat # H00004354-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MPP1 monoclonal antibody (M02), clone 1C3-1D11 (Cat # H00004354-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (77 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant MPP1.
Immunogen
MPP1 (AAH02392, 1 a.a. ~ 466 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MTLKASEGESGGSMHTALSDLYLEHLLQKRSRPEAVSHPLNTVTEDMYTNGSPAPGSPAQVKGQEVRKVRLIQFEKVTEEPMGITLKLNEKQSCTVARILHGGMIHRQGSLHVGDEILEINGTNVTNHSVDQLQKAMKETKGMISLKVIPNQQSRLPALQMFMRAQFDYDPKKDNLIPCKEAGLKFATGDIIQIINKDDSNWWQGRVEGSSKESAGLIPSPELQEWRVASMAQSAPSEAPSCSPFGKKKKYKDKYLAKHSSIFDQLDVVSYEEVVRLPAFKRKTLVLIGASGVGRSHIKNALLSQNPEKFVYPVPYTTRPPRKSEEDGKEYHFISTEEMTRNISANEFLEFGSYQGNMFGTKFETVHQIHKQNKIAILDIEPQTLKIVRTAELSPFIVFIAPTDQGTQTEALQQLQKDSEAIRSQYAHYFDLSLVNNGVDETLKKLQEAFDQACSSPQWVPVSWVY
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (77 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
MPP1 monoclonal antibody (M02), clone 1E11-1G11 Western Blot analysis of MPP1 expression in K-562 ( Cat # L009V1 ).Western Blot (Transfected lysate)
Western Blot analysis of MPP1 expression in transfected 293T cell line by MPP1 monoclonal antibody (M02), clone 1C3-1D11.
Lane 1: MPP1 transfected lysate(52.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MPP1 is approximately 3ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of MPP1 over-expressed 293 cell line, cotransfected with MPP1 Validated Chimera RNAi ( Cat # H00004354-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MPP1 monoclonal antibody (M02), clone 1C3-1D11 (Cat # H00004354-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — MPP1
Entrez GeneID
4354GeneBank Accession#
BC002392Protein Accession#
AAH02392Gene Name
MPP1
Gene Alias
AAG12, DXS552, DXS552E, EMP55, MRG1, PEMP
Gene Description
membrane protein, palmitoylated 1, 55kDa
Omim ID
305360Gene Ontology
HyperlinkGene Summary
Palmitoylated membrane protein 1 is the prototype of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. Palmitoylated membrane protein 1 contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction. [provided by RefSeq
Other Designations
OTTHUMP00000026054|aging-associated gene 12|erythrocyte membrane protein p55|membrane protein, palmitoylated 1 (55kD)|palmitoylated membrane protein 1
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