MPP1 monoclonal antibody (M01), clone 1E11-1G11
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More Files
- More Functions
Western Blot (Tissue lysate)
MPP1 monoclonal antibody (M01), clone 1E11-1G11. Western Blot analysis of MPP1 expression in human placenta.
Western Blot (Transfected lysate)
Western Blot analysis of MPP1 expression in transfected 293T cell line by MPP1 monoclonal antibody (M01), clone 1E11-1G11.
Lane 1: MPP1 transfected lysate (Predicted MW: 52.3 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of MPP1 transfected lysate using anti-MPP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MPP1 monoclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MPP1 is approximately 1ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to MPP1 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (77 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant MPP1.
Immunogen
MPP1 (AAH02392, 1 a.a. ~ 466 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MTLKASEGESGGSMHTALSDLYLEHLLQKRSRPEAVSHPLNTVTEDMYTNGSPAPGSPAQVKGQEVRKVRLIQFEKVTEEPMGITLKLNEKQSCTVARILHGGMIHRQGSLHVGDEILEINGTNVTNHSVDQLQKAMKETKGMISLKVIPNQQSRLPALQMFMRAQFDYDPKKDNLIPCKEAGLKFATGDIIQIINKDDSNWWQGRVEGSSKESAGLIPSPELQEWRVASMAQSAPSEAPSCSPFGKKKKYKDKYLAKHSSIFDQLDVVSYEEVVRLPAFKRKTLVLIGASGVGRSHIKNALLSQNPEKFVYPVPYTTRPPRKSEEDGKEYHFISTEEMTRNISANEFLEFGSYQGNMFGTKFETVHQIHKQNKIAILDIEPQTLKIVRTAELSPFIVFIAPTDQGTQTEALQQLQKDSEAIRSQYAHYFDLSLVNNGVDETLKKLQEAFDQACSSPQWVPVSWVY
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (77 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
MPP1 monoclonal antibody (M01), clone 1E11-1G11. Western Blot analysis of MPP1 expression in human placenta.Western Blot (Transfected lysate)
Western Blot analysis of MPP1 expression in transfected 293T cell line by MPP1 monoclonal antibody (M01), clone 1E11-1G11.
Lane 1: MPP1 transfected lysate (Predicted MW: 52.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of MPP1 transfected lysate using anti-MPP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MPP1 monoclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MPP1 is approximately 1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to MPP1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — MPP1
Entrez GeneID
4354GeneBank Accession#
BC002392Protein Accession#
AAH02392Gene Name
MPP1
Gene Alias
AAG12, DXS552, DXS552E, EMP55, MRG1, PEMP
Gene Description
membrane protein, palmitoylated 1, 55kDa
Omim ID
305360Gene Ontology
HyperlinkGene Summary
Palmitoylated membrane protein 1 is the prototype of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. Palmitoylated membrane protein 1 contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction. [provided by RefSeq
Other Designations
OTTHUMP00000026054|aging-associated gene 12|erythrocyte membrane protein p55|membrane protein, palmitoylated 1 (55kD)|palmitoylated membrane protein 1
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Interactome
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Publication Reference
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MPP1 Determines the Mobility of Flotillins and Controls the Confinement of Raft-Associated Molecules.
Agnieszka Biernatowska, Karolina Wójtowicz, Tomasz Trombik, Aleksander F Sikorski, Aleksander Czogalla.
Cells 2022 Jan; 11(3):311.
Application：IF, Human, HEL cells .
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The microdomain-organizing protein MPP1 is required for insulin-stimulated activation of H-Ras.
Podkalicka J, Biernatowska A, Olszewska P, Tabaczar S, Sikorski AF.
Oncotarget 2018 Apr; 9(26):18410.
Application：WB, Human, MPP1 KnD, SC cultures.
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MPP1 Determines the Mobility of Flotillins and Controls the Confinement of Raft-Associated Molecules.
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