MPL (Human) IP-WB Antibody Pair
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Specifications
Product Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity
Human
Interspecies Antigen Sequence
Mouse (81%); Rat (79%)
Quality Control Testing
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of MPL transfected lysate using rabbit polyclonal anti-MPL and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-MPL.
Supplied Product
Antibody pair set content:
1. Antibody pair for IP: rabbit polyclonal anti-MPL (300 ul)
2. Antibody pair for WB: mouse purified polyclonal anti-MPL (50 ug)
Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
Immunoprecipitation-Western Blot
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Gene Info — MPL
Entrez GeneID
4352Gene Name
MPL
Gene Alias
C-MPL, CD110, MPLV, TPOR
Gene Description
myeloproliferative leukemia virus oncogene
Gene Ontology
HyperlinkGene Summary
In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated. [provided by RefSeq
Other Designations
OTTHUMP00000008582|thrombopoietin receptor
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