MPI 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MPI transfected lysate.
Western Blot
Lane 1: MPI transfected lysate ( 46.7 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MPI full-length
Host
Human
Theoretical MW (kDa)
46.7
Interspecies Antigen Sequence
Mouse (85); Rat (86)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MPI antibody (H00004351-B01) by Western Blots.
SDS-PAGE Gel
MPI transfected lysate.
Western Blot
Lane 1: MPI transfected lysate ( 46.7 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MPI
Entrez GeneID
4351GeneBank Accession#
NM_002435.1Protein Accession#
-Gene Name
MPI
Gene Alias
FLJ39201, PMI, PMI1
Gene Description
mannose phosphate isomerase
Gene Ontology
HyperlinkGene Summary
Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. [provided by RefSeq
Other Designations
Mannosephosphate isomerase (phosphomannose isomerase 1)|mannose-6- phosphate isomerase
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Interactome
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Pathway
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Disease
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