MITF 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MITF transfected lysate.
Western Blot
Lane 1: MITF transfected lysate ( 58.2 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MITF full-length
Host
Human
Theoretical MW (kDa)
58.2
Interspecies Antigen Sequence
Mouse (93); Rat (93)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MITF antibody (H00004286-D01P) by Western Blots.
SDS-PAGE Gel
MITF transfected lysate.
Western Blot
Lane 1: MITF transfected lysate ( 58.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MITF
Entrez GeneID
4286GeneBank Accession#
NM_198159.1Protein Accession#
NP_937802.1Gene Name
MITF
Gene Alias
MI, WS2A, bHLHe32
Gene Description
microphthalmia-associated transcription factor
Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000195123|OTTHUMP00000195140|homolog of mouse microphthalmia
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Interactome
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Pathway
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Disease
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