CD99 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CD99 transfected lysate.
Western Blot
Lane 1: CD99 transfected lysate ( 20.46 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CD99 full-length
Host
Human
Theoretical MW (kDa)
20.46
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CD99 antibody (H00004267-B01) by Western Blots.
SDS-PAGE Gel
CD99 transfected lysate.
Western Blot
Lane 1: CD99 transfected lysate ( 20.46 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CD99
Entrez GeneID
4267GeneBank Accession#
NM_002414.3Protein Accession#
NP_002405.1Gene Name
CD99
Gene Alias
MIC2, MIC2X, MIC2Y
Gene Description
CD99 molecule
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
CD99 antigen|E2 antigen|MIC2 (monoclonal antibody 12E7)|OTTHUMP00000022840|T-cell surface glycoprotein E2|antigen identified by monoclonal 12E7, Y homolog|antigen identified by monoclonal antibodies 12E7, F21 and O13|surface antigen MIC2
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Interactome
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Pathway
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Disease
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