CD99 (Human) Matched Antibody Pair
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Sandwich ELISA detection sensitivity ranging from approximately 2187x to 9x dilution of the CD99 293T overexpression lysate (non-denatured).
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Specification
Product Description
This antibody pair set comes with a matched antibody pair to detect and quantify the protein level of human CD99.
Reactivity
Human
Quality Control Testing
Standard curve using CD99 293T overexpression lysate (non-denatured) as an analyte.
Sandwich ELISA detection sensitivity ranging from approximately 2187x to 9x dilution of the CD99 293T overexpression lysate (non-denatured).
Supplied Product
Antibody pair set content:
1. Capture antibody: mouse monoclonal anti-CD99, IgG2a Kappa (100 ug)
2. Detection antibody: rabbit purified polyclonal anti-CD99 (50 ug)
*Reagents are sufficient for at least 3-5 x 96 well plates using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
ELISA Pair (Transfected lysate)
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Gene Info — CD99
Entrez GeneID
4267Gene Name
CD99
Gene Alias
MIC2, MIC2X, MIC2Y
Gene Description
CD99 molecule
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
CD99 antigen|E2 antigen|MIC2 (monoclonal antibody 12E7)|OTTHUMP00000022840|T-cell surface glycoprotein E2|antigen identified by monoclonal 12E7, Y homolog|antigen identified by monoclonal antibodies 12E7, F21 and O13|surface antigen MIC2
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Interactome
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Pathway
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Disease
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Publication Reference
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Ectodomain shedding of CD99 within highly conserved regions is mediated by the metalloprotease meprin β and promotes transendothelial cell migration.
Bedau T, Peters F, Prox J, Arnold P, Schmidt F, Finkernagel M, Köllmann S, Wichert R, Otte A, Ohler A, Stirnberg M, Lucius R, Koudelka T, Tholey A, Biasin V, Pietrzik CU, Kwapiszewska G, Becker-Pauly C.
FASEB Journal 2016 Dec; 31(3):1226.
Application：ELISA, Quant, Human, HEK 293 cells.
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Ectodomain shedding of CD99 within highly conserved regions is mediated by the metalloprotease meprin β and promotes transendothelial cell migration.
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