MGST1 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MGST1 full-length
Host
Human
Theoretical MW (kDa)
17.6
Interspecies Antigen Sequence
Mouse (83); Rat (83)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MGST1 antibody (H00004257-D01P) by Western Blots.
SDS-PAGE Gel
MGST1 transfected lysate.
Western Blot
Lane 1: MGST1 transfected lysate ( 17.60 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MGST1
Entrez GeneID
4257GeneBank Accession#
NM_020300.3Protein Accession#
NP_064696.1Gene Name
MGST1
Gene Alias
GST12, MGC14525, MGST, MGST-I
Gene Description
microsomal glutathione S-transferase 1
Omim ID
138330Gene Ontology
HyperlinkGene Summary
The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, two of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. Other family members, demonstrating glutathione S-transferase and peroxidase activities, are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. This gene encodes a protein that catalyzes the conjugation of glutathione to electrophiles and the reduction of lipid hydroperoxides. This protein is localized to the endoplasmic reticulum and outer mitochondrial membrane where it is thought to protect these membranes from oxidative stress. Four transcript variants of this gene encode one protein isoform. [provided by RefSeq
Other Designations
glutathione S-transferase 12
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Interactome
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