MGAT1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MGAT1 transfected lysate.
Western Blot
Lane 1: MGAT1 transfected lysate ( 49.06 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MGAT1 full-length
Host
Human
Theoretical MW (kDa)
49.06
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MGAT1 antibody (H00004245-B01) by Western Blots.
SDS-PAGE Gel
MGAT1 transfected lysate.
Western Blot
Lane 1: MGAT1 transfected lysate ( 49.06 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MGAT1
Entrez GeneID
4245GeneBank Accession#
NM_002406.2Protein Accession#
NP_002397.1Gene Name
MGAT1
Gene Alias
GLCNAC-TI, GLCT1, GLYT1, GNT-1, GNT-I, MGAT
Gene Description
mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
Omim ID
160995Gene Ontology
HyperlinkGene Summary
There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase|OTTHUMP00000161546
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Interactome
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Pathway
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Disease
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