MFNG 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
MFNG transfected lysate.
Western Blot
Lane 1: MFNG transfected lysate ( 36.2 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MFNG full-length
Host
Human
Theoretical MW (kDa)
36.2
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MFNG antibody (H00004242-B01) by Western Blots.
SDS-PAGE Gel
MFNG transfected lysate.
Western Blot
Lane 1: MFNG transfected lysate ( 36.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MFNG
Entrez GeneID
4242GeneBank Accession#
NM_002405.2Protein Accession#
NP_002396.2Gene Name
MFNG
Gene Alias
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Gene Description
MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Omim ID
602577Gene Ontology
HyperlinkGene Summary
This gene is a member of the fringe gene family which also includes Radical and Lunatic fringe. They all encode evolutionarily conserved secreted proteins that act in the Notch receptor pathway to demarcate boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, fringe proteins have a fucose-specific beta1,3 N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. [provided by RefSeq
Other Designations
O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase|OTTHUMP00000043697|OTTHUMP00000043698|OTTHUMP00000043700|beta-1,3-N-acetylglucosaminyltransferase manic fringe|manic fringe homolog
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Interactome
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Pathway
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Disease
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