MAP3K3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MAP3K3 transfected lysate.
Western Blot
Lane 1: MAP3K3 transfected lysate ( 10.01 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MAP3K3 full-length
Host
Human
Theoretical MW (kDa)
10.01
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MAP3K3 antibody (H00004215-B02) by Western Blots.
SDS-PAGE Gel
MAP3K3 transfected lysate.
Western Blot
Lane 1: MAP3K3 transfected lysate ( 10.01 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MAP3K3
Entrez GeneID
4215GeneBank Accession#
BC010464.1Protein Accession#
AAH10464.1Gene Name
MAP3K3
Gene Alias
MAPKKK3, MEKK3
Gene Description
mitogen-activated protein kinase kinase kinase 3
Omim ID
602539Gene Ontology
HyperlinkGene Summary
This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
MAP/ERK kinase kinase 3|MAPK/ERK kinase kinase 3
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Interactome
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Pathway
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Disease
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