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MAP3K3 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00004215-T02
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-MAP3K3 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 10.01
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-MAP3K3 antibody (H00004215-B02) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00004215-T02
    MAP3K3 transfected lysate.
    Western Blot
    QC Testing of H00004215-T02
    Lane 1: MAP3K3 transfected lysate ( 10.01 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 4215
  • Gene Name:
  • MAP3K3
  • Gene Alias:
  • MAPKKK3,MEKK3
  • Gene Description:
  • mitogen-activated protein kinase kinase kinase 3
  • Gene Summary:
  • This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
  • Other Designations:
  • MAP/ERK kinase kinase 3,MAPK/ERK kinase kinase 3
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