Proximity Ligation Analysis of protein-protein interactions between MAPK3 and MAP3K1. HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-MAP3K1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
MAP3K, or MEK kinase, is a serine/threonine kinase that occupies a pivotal role in a network of phosphorylating enzymes integrating cellular responses to a number of mitogenic and metabolic stimuli, including insulin (MIM 176730) and many growth factors.[supplied by OMIM