ME1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ME1 full-length
Host
Human
Theoretical MW (kDa)
64.1
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ME1 antibody (H00004199-D01P) by Western Blots.
SDS-PAGE Gel
ME1 transfected lysate.
Western Blot
Lane 1: ME1 transfected lysate ( 64.10 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ME1
Entrez GeneID
4199GeneBank Accession#
NM_002395.3Protein Accession#
NP_002386.1Gene Name
ME1
Gene Alias
HUMNDME, MES
Gene Description
malic enzyme 1, NADP(+)-dependent, cytosolic
Omim ID
154250Gene Ontology
HyperlinkGene Summary
This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq
Other Designations
Malic enzyme, cytoplasmic|NADP-dependent malic enzyme|OTTHUMP00000016792|cytosolic malic enzyme 1|malate dehydrogenase|malic enzyme 1, soluble|pyruvic-malic carboxylase
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of terpenoids and steroids
- Carbon fixation in photosynthetic organisms
- Metabolic pathways
- PPAR signaling pathway
- Pyruvate metabolism
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Disease
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