ME1 purified MaxPab rabbit polyclonal antibody (D01P)
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of ME1 expression in transfected 293T cell line (H00004199-T01) by ME1 MaxPab polyclonal antibody.
Lane 1: ME1 transfected lysate(64.10 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to ME1 on HeLa cell. [antibody concentration 30 ug/ml]
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Specification
Product Description
Rabbit polyclonal antibody raised against a full-length human ME1 protein.
Immunogen
ME1 (NP_002386.1, 1 a.a. ~ 572 a.a) full-length human protein.
Sequence
MEPEAPRRRHTHQRGYLLTRNPHLNKDLAFTLEERQQLNIHGLLPPSFNSQEIQVLRVVKNFEHLNSDFDRYLLLMDLQDRNEKLFYRVLTSDIEKFMPIVYTPTVGLACQQYSLVFRKPRGLFITIHDRGHIASVLNAWPEDVIKAIVVTDGERILGLGDLGCNGMGIPVGKLALYTACGGMNPQECLPVILDVGTENEELLKDPLYIGLRQRRVRGSEYDDFLDEFMEAVSSKYGMNCLIQFEDFANVNAFRLLNKYRNQYCTFNDDIQGTASVAVAGLLAALRITKNKLSDQTILFQGAGEAALGIAHLIVMALEKEGLPKEKAIKKIWLVDSKGLIVKGRASLTQEKEKFAHEHEEMKNLEAIVQEIKPTALIGVAAIGGAFSEQILKDMAAFNERPIIFALSNPTSKAECSAEQCYKITKGRAIFASGSPFDPVTLPNGQTLYPGQGNNSYVFPGVALGVVACGLRQITDNIFLTTAEVIAQQVSDKHLEEGRLYPPLNTIRDVSLKIAEKIVKDAYQEKTATVYPEPQNKEAFVRSQMYSTDYDQILPDCYSWPEEVQKIQTKVDQ
Host
Rabbit
Reactivity
Human
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of ME1 expression in transfected 293T cell line (H00004199-T01) by ME1 MaxPab polyclonal antibody.
Lane 1: ME1 transfected lysate(64.10 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to ME1 on HeLa cell. [antibody concentration 30 ug/ml] -
Gene Info — ME1
Entrez GeneID
4199GeneBank Accession#
NM_002395.3Protein Accession#
NP_002386.1Gene Name
ME1
Gene Alias
HUMNDME, MES
Gene Description
malic enzyme 1, NADP(+)-dependent, cytosolic
Omim ID
154250Gene Ontology
HyperlinkGene Summary
This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq
Other Designations
Malic enzyme, cytoplasmic|NADP-dependent malic enzyme|OTTHUMP00000016792|cytosolic malic enzyme 1|malate dehydrogenase|malic enzyme 1, soluble|pyruvic-malic carboxylase
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of terpenoids and steroids
- Carbon fixation in photosynthetic organisms
- Metabolic pathways
- PPAR signaling pathway
- Pyruvate metabolism
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Disease
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