MDFI (Human) Recombinant Protein (Q01)
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Specification
Product Description
Human MDFI partial ORF ( NP_005577, 33 a.a. - 110 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
PGLEVVTGSTHPAEAAPEEGSLEEAATPMPQGNGPGIPQGLDSTDLDVPTEAVTCQPQGNPLGCTPLLPNDSGHPSEL
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
34.32
Interspecies Antigen Sequence
Mouse (69); Rat (76)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — MDFI
Entrez GeneID
4188GeneBank Accession#
NM_005586Protein Accession#
NP_005577Gene Name
MDFI
Gene Alias
I-MF, I-mfa
Gene Description
MyoD family inhibitor
Omim ID
604971Gene Ontology
HyperlinkGene Summary
This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. [provided by RefSeq
Other Designations
OTTHUMP00000016375|OTTHUMP00000196414|inhibitor of MyoD family a
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