MDFI monoclonal antibody (M07), clone 3B4
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of MDFI expression in transfected 293T cell line by MDFI monoclonal antibody (M07), clone 3B4.
Lane 1: MDFI transfected lysate(25.029 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of monoclonal antibody to MDFI on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (34.32 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant MDFI.
Immunogen
MDFI (NP_005577, 33 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
PGLEVVTGSTHPAEAAPEEGSLEEAATPMPQGNGPGIPQGLDSTDLDVPTEAVTCQPQGNPLGCTPLLPNDSGHPSEL
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (69); Rat (76)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (34.32 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of MDFI expression in transfected 293T cell line by MDFI monoclonal antibody (M07), clone 3B4.
Lane 1: MDFI transfected lysate(25.029 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to MDFI on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — MDFI
Entrez GeneID
4188GeneBank Accession#
NM_005586Protein Accession#
NP_005577Gene Name
MDFI
Gene Alias
I-MF, I-mfa
Gene Description
MyoD family inhibitor
Omim ID
604971Gene Ontology
HyperlinkGene Summary
This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. [provided by RefSeq
Other Designations
OTTHUMP00000016375|OTTHUMP00000196414|inhibitor of MyoD family a
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