MCM4 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MCM4 transfected lysate.
Western Blot
Lane 1: MCM4 transfected lysate ( 96.6 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MCM4 full-length
Host
Human
Theoretical MW (kDa)
96.6
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MCM4 antibody (H00004173-B01) by Western Blots.
SDS-PAGE Gel
MCM4 transfected lysate.
Western Blot
Lane 1: MCM4 transfected lysate ( 96.6 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MCM4
Entrez GeneID
4173GeneBank Accession#
BC031061Protein Accession#
AAH31061Gene Name
MCM4
Gene Alias
CDC21, CDC54, MGC33310, P1-CDC21, hCdc21
Gene Description
minichromosome maintenance complex component 4
Omim ID
602638Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq
Other Designations
DNA replication licensing factor MCM4|MCM4 minichromosome maintenance deficient 4|homolog of S. pombe cell devision cycle 21|minichromosome maintenance deficient 4
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