MCC monoclonal antibody (M01), clone 1A7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of MCC expression in transfected 293T cell line by MCC monoclonal antibody (M01), clone 1A7.
Lane 1: MCC transfected lysate(93.1 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MCC is approximately 0.03ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of MCC over-expressed 293 cell line, cotransfected with MCC Validated Chimera RNAi ( Cat # H00004163-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MCC monoclonal antibody (M01), clone 1A7 (Cat # H00004163-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (36.63 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant MCC.
Immunogen
MCC (AAH09279, 31 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
DIVELNKRLQQTERERDLLEKKLAKAQCEQSHLMREHEDVQERTTLRYEERITELHSVIAELNKKIDRLQGTTIREEDEYSELRSELSQSQHEVNEDSRS
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of MCC expression in transfected 293T cell line by MCC monoclonal antibody (M01), clone 1A7.
Lane 1: MCC transfected lysate(93.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MCC is approximately 0.03ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of MCC over-expressed 293 cell line, cotransfected with MCC Validated Chimera RNAi ( Cat # H00004163-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MCC monoclonal antibody (M01), clone 1A7 (Cat # H00004163-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — MCC
Entrez GeneID
4163GeneBank Accession#
BC009279Protein Accession#
AAH09279Gene Name
MCC
Gene Alias
DKFZp762O1615, FLJ38893, FLJ46755, MCC1
Gene Description
mutated in colorectal cancers
Omim ID
159350Gene Ontology
HyperlinkGene Summary
This gene is a candidate colorectal tumor suppressor gene that is thought to negatively regulate cell cycle progression. The orthologous gene in the mouse expresses a phosphoprotein associated with the plasma membrane and membrane organelles, and overexpression of the mouse protein inhibits entry into S phase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
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Interactome
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Disease
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