MCAM (Human) Recombinant Protein
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More Files
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Specification
Product Description
Purified MCAM (NP_006491.2, 24 a.a. - 646 a.a.) human recombinant protein with His-Flag-StrepII tag at N-terminus expressed in human cells.
Transfected Cell Line
Human HEK293H cells
Sequence
VPGEAEQPAPELVEVEVGSTALLKCGLSQSQGNLSHVDWFSVHKEKRTLIFRVRQGQGQSEPGEYEQRLSLQDRGATLALTQVTPQDERIFLCQGKRPRSQEYRIQLRVYKAPEEPNIQVNPLGIPVNSKEPEEVATCVGRNGYPIPQVIWYKNGRPLKEEKNRVHIQSSQTVESSGLYTLQSILKAQLVKEDKDAQFYCELNYRLPSGNHMKESREVTVPVFYPTEKVWLEVEPVGMLKEGDRVEIRCLADGNPPPHFSISKQNPSTREAEEETTNDNGVLVLEPARKEHSGRYECQGLDLDTMISLLSEPQELLVNYVSDVRVSPAAPERQEGSSLTLTCEAESSQDLEFQWLREETGQVLERGPVLQLHDLKREAGGGYRCVASVPSIPGLNRTQLVNVAIFGPPWMAFKERKVWVKENMVLNLSCEASGHPRPTISWNVNGTASEQDQDPQRVLSTLNVLVTPELLETGVECTASNDLGKNTSILFLELVNLTTLTPDSNTTTGLSTSTASPHTRANSTSTERKLPEPESRGVVIVAVIVCILVLAVLGAVLYFLYKKGKLPCRRSGKQEITLPPSRKSELVVEVKSDKLPEEMGLLQGSSGDKRAPGDQGEKYIDLRH
Host
Human
Theoretical MW (kDa)
71.61
Interspecies Antigen Sequence
Mouse (76); Rat (75)
Form
Liquid
Preparation Method
Transfection of pSuper-MCAM plasmid into HEK293H cell, and the expressed protein was purified by Strep-Tactin affinity column.
Purification
Strep-Tactin affinity columns
Concentration
≥ 10 ug/ml
Quality Control Testing
SDS-PAGE and Western Blot
SDS-PAGE Gel
Western Blot
Storage Buffer
100 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM EDTA, and 5 mM desthiobiotin.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Enzyme-linked Immunoabsorbent Assay
SDS-PAGE
Protein Interaction
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Gene Info — MCAM
Entrez GeneID
4162GeneBank Accession#
NM_006500.2Protein Accession#
NP_006491.2Gene Name
MCAM
Gene Alias
CD146, MUC18
Gene Description
melanoma cell adhesion molecule
Omim ID
155735Gene Ontology
HyperlinkOther Designations
Gicerin|melanoma adhesion molecule
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Interactome
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