MB monoclonal antibody (M13), clone 4F8
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of MB expression in transfected 293T cell line by MB monoclonal antibody (M13), clone 4F8.
Lane 1: MB transfected lysate(17.2 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MB is 0.03 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to MB on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (36.52 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant MB.
Immunogen
MB (NP_005359.1, 2 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
GLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKI
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.52 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of MB expression in transfected 293T cell line by MB monoclonal antibody (M13), clone 4F8.
Lane 1: MB transfected lysate(17.2 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MB is 0.03 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to MB on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — MB
Entrez GeneID
4151GeneBank Accession#
NM_005368.2Protein Accession#
NP_005359.1Gene Name
MB
Gene Alias
MGC13548, PVALB
Gene Description
myoglobin
Omim ID
160000Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq
Other Designations
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