MAT1A monoclonal antibody (M01), clone 4D11
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of MAT1A expression in transfected 293T cell line by MAT1A monoclonal antibody (M01), clone 4D11.
Lane 1: MAT1A transfected lysate(43.6 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of MAT1A transfected lysate using anti-MAT1A monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MAT1A monoclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MAT1A is 1 ng/ml as a capture antibody.
Western Blot detection against Immunogen (69.19 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant MAT1A.
Immunogen
MAT1A (AAH18359, 1 a.a. ~ 395 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MNGPVDGLCDHSLSEGVFMFTSESVGEGHPDKICDQISDAVLDAHLKQDPNAKVACETVCKTGMVLLCGEITSMAMVDYQRVVRDTIKHIGYDDSAKGFDFKTCNVLVALEQQSPDIAQCVHLDRNEEDVGAGDQGLMFGYATDETEECMPLTIILAHKLNARMADLRRSGLLPWLRPDSKTQVTVQYMQDNGAVIPVRIHTIVISVQHNEDITLEEMRRALKEQVIRAVVPAKYLDEDTVYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAEPLSISIFTYGTSQKTERELLDVVHKNFDLRPGVIVRDLDLKKPIYQKTACYGHFGRSEFPWEVPRKLVF
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (69.19 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of MAT1A expression in transfected 293T cell line by MAT1A monoclonal antibody (M01), clone 4D11.
Lane 1: MAT1A transfected lysate(43.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of MAT1A transfected lysate using anti-MAT1A monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MAT1A monoclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MAT1A is 1 ng/ml as a capture antibody.ELISA
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Gene Info — MAT1A
Entrez GeneID
4143GeneBank Accession#
BC018359Protein Accession#
AAH18359Gene Name
MAT1A
Gene Alias
MAT, MATA1, SAMS, SAMS1
Gene Description
methionine adenosyltransferase I, alpha
Gene Ontology
HyperlinkGene Summary
This gene catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. The encoded protein is found as a homotetramer (MAT I) or a homodimer (MAT III) whereas a third form, MAT II (gamma), is encoded by the MAT2A gene. Mutations in this gene are associated with methionine adenosyltransferase deficiency. [provided by RefSeq
Other Designations
OTTHUMP00000019971|S-adenosylmethionine synthetase
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Interactome
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Pathway
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Disease
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