MANBA 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MANBA full-length
Host
Human
Theoretical MW (kDa)
96.8
Interspecies Antigen Sequence
Mouse (76); Rat (75)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MANBA antibody (H00004126-B01) by Western Blots.
SDS-PAGE Gel
MANBA transfected lysate.
Western Blot
Lane 1: MANBA transfected lysate ( 96.8 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MANBA
Entrez GeneID
4126GeneBank Accession#
BC015743.1Protein Accession#
AAH15743.1Gene Name
MANBA
Gene Alias
MANB1
Gene Description
mannosidase, beta A, lysosomal
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the glycosyl hydrolase 2 family. The encoded protein localizes to the lysosome where it is the final exoglycosidase in the pathway for N-linked glycoprotein oligosaccharide catabolism. Mutations in this gene are associated with beta-mannosidosis, a lysosomal storage disease that has a wide spectrum of neurological involvement. [provided by RefSeq
Other Designations
mannanase|mannase
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Interactome
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Pathway
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Disease
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