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MAN2B1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00004125-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-MAN2B1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 111.32
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-MAN2B1 antibody (H00004125-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00004125-T01
    MAN2B1 transfected lysate.
    Western Blot
    QC Testing of H00004125-T01
    Lane 1: MAN2B1 transfected lysate ( 111.32 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 4125
  • Gene Name:
  • MAN2B1
  • Gene Alias:
  • LAMAN,MANB
  • Gene Description:
  • mannosidase, alpha, class 2B, member 1
  • Gene Summary:
  • This gene encodes an enzyme that hydrolyzes terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. Its activity is necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover and it is member of family 38 of glycosyl hydrolases. The full length protein is processed in two steps. First, a 49 aa leader sequence is cleaved off and the remainder of the protein is processed into 3 peptides of 70 kDa, 42 kDa (D) and 13/15 kDa (E). Next, the 70 kDa peptide is further processed into three peptides (A, B and C). The A, B and C peptides are disulfide-linked. Defects in this gene have been associated with lysosomal alpha-mannosidosis. [provided by RefSeq
  • Other Designations:
  • lysosomal acid alpha-mannosidase,mannosidase, alpha B, lysosomal
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