MAGEA4 monoclonal antibody (M01), clone 3D12
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More Files
- More Functions
Western Blot (Cell lysate)
MAGEA4 monoclonal antibody (M01), clone 3D12 Western Blot analysis of MAGEA4 expression in Hela S3 NE ( Cat # L013V3 ).
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MAGEA4 is approximately 0.3ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to MAGEA4 on HeLa cell. [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant MAGEA4.
Immunogen
MAGEA4 (NP_002353, 98 a.a. ~ 171 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
TSPDAESLFREALSNKVDELAHFLLRKYRAKELVTKAEMLERVIKNYKRCFPVIFGKASESLKMIFGIDVKEVD
Host
Mouse
Reactivity
Human
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
MAGEA4 monoclonal antibody (M01), clone 3D12 Western Blot analysis of MAGEA4 expression in Hela S3 NE ( Cat # L013V3 ).Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MAGEA4 is approximately 0.3ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to MAGEA4 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — MAGEA4
Entrez GeneID
4103GeneBank Accession#
NM_002362Protein Accession#
NP_002353Gene Name
MAGEA4
Gene Alias
MAGE-41, MAGE-X2, MAGE4, MAGE4A, MAGE4B, MGC21336
Gene Description
melanoma antigen family A, 4
Omim ID
300175Gene Ontology
HyperlinkGene Summary
This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. At least four variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000025889|OTTHUMP00000025890|melanoma-associated antigen 4
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Interactome
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Publication Reference
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Heteroclitic serological response in esophageal and prostate cancer patients after NY?ESO?1 protein vaccination.
Kawada J, Wada H, Isobe M, Gnjatic S, Nishikawa H, Jungbluth AA, Okazaki N, Uenaka A, Nakamura Y, Fujiwara S, Mizuno N, Saika T, Ritter E, Yamasaki M, Miyata H, Ritter G, Murphy R, Venhaus R, Pan L, Old LJ, Doki Y, Nakayama E.
International Journal of Cancer 2012 Feb; 30(3):584.
Application：WB, Mouse, CMS5a cells.
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Heteroclitic serological response in esophageal and prostate cancer patients after NY?ESO?1 protein vaccination.
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