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SMAD1 293T Cell Transient Overexpression Lysate(Denatured)   

  • Catalog # : H00004086-T01
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  • 100 uL
  • USD $ 239
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-SMAD1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 52.3
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-SMAD1 antibody (H00004086-D01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00004086-T01
    SMAD1 transfected lysate.
    Western Blot
    QC Testing of H00004086-T01
    Lane 1: SMAD1 transfected lysate ( 52.30 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 4086
  • Gene Name:
  • SMAD1
  • Gene Alias:
  • BSP1,JV4-1,JV41,MADH1,MADR1
  • Gene Description:
  • SMAD family member 1
  • Gene Summary:
  • The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq
  • Other Designations:
  • MAD, mothers against decapentaplegic homolog 1,Mad-related protein 1,SMAD, mothers against DPP homolog 1,Sma- and Mad-related protein 1,TGF-beta signaling protein 1,mothers against DPP homolog 1,transforming growth factor-beta signaling protein 1
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