LIPA monoclonal antibody (M01), clone 1F9
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of LIPA expression in transfected 293T cell line by LIPA monoclonal antibody (M01), clone 1F9.
Lane 1: LIPA transfected lysate(45 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of LIPA transfected lysate using anti-LIPA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LIPA MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged LIPA is approximately 10ng/ml as a capture antibody.
Western Blot detection against Immunogen (69.63 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant LIPA.
Immunogen
LIPA (AAH12287, 1 a.a. ~ 399 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MKMRFLGLVVCLVLWPLHSEGSGGKLTALDPETNMNVSEIISYWGFPSEEYLVETEDGYILCLNRIPHGRKNHSDKGPKPVVFLQHGLLADSSNWVTNLANSSLGFILADAGFDVWMGNSRGNTWSRKHKTLSVSQDEFWAFSYDEMAKYDLPASINFILNKTGQEQVYYVGHSQGTTIGFIAFSQIPELAKRIKMFFALGPVASVAFCTSPMAKLGRLPDHLIKDLFGDKEFLPQSAFLKWLGTHVCTHVILKELCGNLCFLLCGFNERNLNMSRVDVYTTHSPAGTSVQNMLHWSQAVKFQKFQAFDWGSSAKNYFHYNQSYPPTYNVKDMLVPTAVWSGGHDWLADVYDVNILLTQITNLVFHESIPEWEHLDFIWGLDAPWRLYNKIINLMRKYQ
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (74); Rat (76)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (69.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of LIPA expression in transfected 293T cell line by LIPA monoclonal antibody (M01), clone 1F9.
Lane 1: LIPA transfected lysate(45 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of LIPA transfected lysate using anti-LIPA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LIPA MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged LIPA is approximately 10ng/ml as a capture antibody.ELISA
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Gene Info — LIPA
Entrez GeneID
3988GeneBank Accession#
BC012287Protein Accession#
AAH12287Gene Name
LIPA
Gene Alias
CESD, LAL
Gene Description
lipase A, lysosomal acid, cholesterol esterase
Omim ID
278000Gene Ontology
HyperlinkGene Summary
This gene encodes lipase A, the lysosomal acid lipase (also known as cholesterol ester hydrolase). This enzyme functions in the lysosome to catalyze the hydrolysis of cholesteryl esters and triglycerides. Mutations in this gene can result in Wolman disease and cholesteryl ester storage disease. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000020066|OTTHUMP00000020068|OTTHUMP00000059643|cholesterol ester hydrolase|lipase A|lysosomal acid lipase|sterol esterase
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Interactome
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Pathway
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Disease
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