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LIMK2 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00003985-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-LIMK2 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 75.57
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-LIMK2 antibody (H00003985-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00003985-T01
    LIMK2 transfected lysate.
    Western Blot
    QC Testing of H00003985-T01
    Lane 1: LIMK2 transfected lysate ( 75.57 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 3985
  • Gene Name:
  • LIMK2
  • Gene Alias:
  • -
  • Gene Description:
  • LIM domain kinase 2
  • Gene Summary:
  • There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • -
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