LIG1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-LIG1 full-length
Host
Human
Theoretical MW (kDa)
101.7
Interspecies Antigen Sequence
Mouse (84%)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-LIG1 antibody (H00003978-B01) by Western Blots.
SDS-PAGE Gel
LIG1 transfected lysate.
Western Blot
Lane 1: LIG1 transfected lysate ( 101.70 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — LIG1
Entrez GeneID
3978GeneBank Accession#
NM_000234Protein Accession#
NP_000225.1Gene Name
LIG1
Gene Alias
MGC117397, MGC130025
Gene Description
ligase I, DNA, ATP-dependent
Omim ID
126391Gene Ontology
HyperlinkGene Summary
LIG1 encodes DNA ligase I, with functions in DNA replication and the base excision repair process. Mutations in LIG1 that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. [provided by RefSeq
Other Designations
DNA ligase I
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Interactome
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Pathway
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Disease
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