LDHA 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
LDHA transfected lysate.
Western Blot
Lane 1: LDHA transfected lysate ( 36.7 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-LDHA full-length
Host
Human
Theoretical MW (kDa)
36.7
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-LDHA antibody (H00003939-B01) by Western Blots.
SDS-PAGE Gel
LDHA transfected lysate.
Western Blot
Lane 1: LDHA transfected lysate ( 36.7 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — LDHA
Entrez GeneID
3939GeneBank Accession#
NM_005566.1Protein Accession#
NP_005557.1Gene Name
LDHA
Gene Alias
LDH-M, LDH1, PIG19
Gene Description
lactate dehydrogenase A
Omim ID
150000Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq
Other Designations
L-lactate dehydrogenase A|proliferation-inducing gene 19
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Interactome
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Pathway
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Disease
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