LCK 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
LCK transfected lysate.
Western Blot
Lane 1: LCK transfected lysate ( 61.20 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-LCK full-length
Host
Human
Theoretical MW (kDa)
61.2
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-LCK antibody (H00003932-D01P) by Western Blots.
SDS-PAGE Gel
LCK transfected lysate.
Western Blot
Lane 1: LCK transfected lysate ( 61.20 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — LCK
Entrez GeneID
3932GeneBank Accession#
BC013200.1Protein Accession#
AAH13200.1Gene Name
LCK
Gene Alias
YT16, p56lck, pp58lck
Gene Description
lymphocyte-specific protein tyrosine kinase
Omim ID
153390Gene Ontology
HyperlinkGene Summary
This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. [provided by RefSeq
Other Designations
T-lymphocyte specific protein tyrosine kinase p56lck|p56(LSTRA) protein-tyrosine kinase|protein tyrosine kinase|proto-oncogene tyrosine-protein kinase LCK
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Interactome
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