KRT81 monoclonal antibody (M01J), clone 3B10-5B10
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant KRT81.
This product is belong to Cell Culture Grade Antibody (CX Grade).Immunogen
KRT81 (AAH21241, 1 a.a. ~ 202 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MKATVIRHGETLRRTKEEINELNRMIQRLTAEVENAKCQNSKLEAAVAQSEQQGEAALSDARCKLAELEGALQKAKQDMACLIREYQEVMNSKLGLDIEIATYRRLLEGEEQRLCEGIGAVNVCVSSSRGGVVCGDLCVSGSRPVTGSVCSAPCNGNVAVSTGLCAPCGQLNTTCGGGSCGVGSCGISSLGVGSCGSSCRKC
Host
Mouse
Reactivity
Human
Preparation Method
Cell Culture Production
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (47.96 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
KRT81 monoclonal antibody (M01J), clone 3B10-5B10. Western Blot analysis of KRT81 expression in A-431.Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to KRT81 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged KRT81 is 0.3 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to KRT81 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — KRT81
Entrez GeneID
3887GeneBank Accession#
BC021241Protein Accession#
AAH21241Gene Name
KRT81
Gene Alias
HB1, Hb-1, KRTHB1, MLN137, ghHkb1, hHAKB2-1
Gene Description
keratin 81
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily, consisting of KRTHB1, KRTHB3, and KRTHB6, is highly related. The other less-related subfamily includes KRTHB2, KRTHB4, and KRTHB5. All hair keratins are expressed in the hair follicle; this hair keratin, as well as KRTHB3 and KRTHB6, is found primarily in the hair cortex. Mutations in this gene and KRTHB6 have been observed in patients with a rare dominant hair disease, monilethrix. [provided by RefSeq
Other Designations
hard keratin, type II, 1|keratin, hair, basic, 1
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