KRT5 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
KRT5 transfected lysate.
Western Blot
Lane 1: KRT5 transfected lysate ( 62.40 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-KRT5 full-length
Host
Human
Theoretical MW (kDa)
62.4
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-KRT5 antibody (H00003852-D01P) by Western Blots.
SDS-PAGE Gel
KRT5 transfected lysate.
Western Blot
Lane 1: KRT5 transfected lysate ( 62.40 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — KRT5
Entrez GeneID
3852GeneBank Accession#
BC024292.1Protein Accession#
AAH24292.1Gene Name
KRT5
Gene Alias
CK5, DDD, EBS2, K5, KRT5A
Gene Description
keratin 5
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq
Other Designations
58 kda cytokeratin|epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types|keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types)|keratin, type II cytoskeletal 5
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Interactome
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Disease
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