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RANBP5 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00003843-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-RANBP5 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 125.6
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-RANBP5 antibody (H00003843-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00003843-T01
    RANBP5 transfected lysate.
    Western Blot
    QC Testing of H00003843-T01
    Lane 1: RANBP5 transfected lysate ( 125.6 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 3843
  • Gene Name:
  • IPO5
  • Gene Alias:
  • DKFZp686O1576,FLJ43041,IMB3,KPNB3,MGC2068,RANBP5
  • Gene Description:
  • importin 5
  • Gene Summary:
  • Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000040733,RAN binding protein 5,Ran_GTP binding protein 5,importin beta-3 subunit,karyopherin (importin) beta 3
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