KPNA1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
KPNA1 transfected lysate.
Western Blot
Lane 1: KPNA1 transfected lysate ( 60.20 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-KPNA1 full-length
Host
Human
Theoretical MW (kDa)
60.2
Interspecies Antigen Sequence
Mouse (98); Rat (99)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-KPNA1 antibody (H00003836-D01P) by Western Blots.
SDS-PAGE Gel
KPNA1 transfected lysate.
Western Blot
Lane 1: KPNA1 transfected lysate ( 60.20 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — KPNA1
Entrez GeneID
3836GeneBank Accession#
NM_002264Protein Accession#
NP_002255.2Gene Name
KPNA1
Gene Alias
IPOA5, NPI-1, RCH2, SRP1
Gene Description
karyopherin alpha 1 (importin alpha 5)
Omim ID
600686Gene Ontology
HyperlinkGene Summary
Recombination activating proteins RAG1 and RAG2 regulate and mediate V(D)J recombination, the process by which genes for immunoglobulins and T-cell receptors are generated. Several other ubiquitously expressed proteins are thought to be recruited in the recombination process. Among these are the genes affected in severe combined immune deficiency and genes involved in ds-DNA break repair. The protein encoded by this gene interacts with RAG1 and may play a role in V(D)J recombination. Two transcript variants, one protein-coding and the other not, have been found for this gene. [provided by RefSeq
Other Designations
importin alpha 5|importin-alpha-S1|karyopherin alpha 1|nucleoprotein interactor 1|recombination activating gene cohort 2
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