KISS1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-KISS1 full-length
Host
Human
Theoretical MW (kDa)
14.7
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-KISS1 antibody (H00003814-D01P) by Western Blots.
SDS-PAGE Gel
KISS1 transfected lysate.
Western Blot
Lane 1: KISS1 transfected lysate ( 14.70 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — KISS1
Entrez GeneID
3814GeneBank Accession#
BC022819.1Protein Accession#
NP_002247.3Gene Name
KISS1
Gene Alias
KiSS-1, METASTIN, MGC39258
Gene Description
KiSS-1 metastasis-suppressor
Omim ID
603286Gene Ontology
HyperlinkGene Summary
This gene is a metastasis suppressor gene that suppresses metastases of melanomas and breast carcinomas without affecting tumorigenicity. The encoded protein may function to inhibit chemotaxis and invasion, attenuating metastasis in malignant melanomas. Studies suggest a putative role in the regulation of events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A polymorphism in the terminal exon of this mRNA results in two protein isoforms. An adenosine present at the polymorphic site represents the third position in a stop codon. When the adenosine is absent, a downstream stop codon is utilized and the encoded protein extends for an additional seven amino acid residues. [provided by RefSeq
Other Designations
OTTHUMP00000034312|kisspeptin|malignant melanoma metastasis-suppressor
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Interactome
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Disease
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