KHK monoclonal antibody (M01), clone 1H6-2B6
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More Files
- More Functions
Western Blot (Tissue lysate)
KHK monoclonal antibody (M01), clone 1H6-2B6. Western Blot analysis of KHK expression in human liver.
Western Blot (Transfected lysate)
Western Blot analysis of KHK expression in transfected 293T cell line by KHK monoclonal antibody (M01), clone 1H6-2B6.
Lane 1: KHK transfected lysate(32.7 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to KHK on formalin-fixed paraffin-embedded human spleen tissue. [antibody concentration 1 ~ 10 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged KHK is approximately 0.3ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of KHK over-expressed 293 cell line, cotransfected with KHK Validated Chimera RNAi ( Cat # H00003795-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with KHK monoclonal antibody (M01), clone 1H6-2B6 (Cat # H00003795-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (58.52 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant KHK.
Immunogen
KHK (AAH06233, 1 a.a. ~ 298 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MEEKQILCVGLVVLDVISLVDKYPKEDSEIRCLSQRWQRGGNASNSCTILSLLGAPCAFMGSMAPGHVADFVLDDLRRYSVDLRYTVFQTTGSVPIATVIINEASGSRTILYYDRSLPDVSATDFEKVDLTQFKWIHIEGRNASEQVKMLQRIDAHNTRQPPEQKIRVSVEVEKPREELFQLFGYGDVVFVSKDVAKHLGFQSAEEALRGLYGRVRKGAVLVCAWAEEGADALGPDGKLLHSDAFPPPRVVDTLGAGDTFNASVIFSLSQGRSVQEALRFGCQVAGKKCGLQGFDGIV
Host
Mouse
Reactivity
Human
Isotype
IgG2a kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (58.52 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
KHK monoclonal antibody (M01), clone 1H6-2B6. Western Blot analysis of KHK expression in human liver.Western Blot (Transfected lysate)
Western Blot analysis of KHK expression in transfected 293T cell line by KHK monoclonal antibody (M01), clone 1H6-2B6.
Lane 1: KHK transfected lysate(32.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to KHK on formalin-fixed paraffin-embedded human spleen tissue. [antibody concentration 1 ~ 10 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged KHK is approximately 0.3ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of KHK over-expressed 293 cell line, cotransfected with KHK Validated Chimera RNAi ( Cat # H00003795-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with KHK monoclonal antibody (M01), clone 1H6-2B6 (Cat # H00003795-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — KHK
Entrez GeneID
3795GeneBank Accession#
BC006233Protein Accession#
AAH06233Gene Name
KHK
Gene Alias
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Gene Description
ketohexokinase (fructokinase)
Omim ID
229800Gene Ontology
HyperlinkGene Summary
This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
ketohexokinase
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Interactome
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Pathway
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Disease
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