JUP monoclonal antibody (M01), clone 2G9
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More Files
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Western Blot (Cell lysate)
JUP monoclonal antibody (M01), clone 2G9 Western Blot analysis of JUP expression in A-431 ( Cat # L015V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of JUP expression in transfected 293T cell line by JUP monoclonal antibody (M01), clone 2G9.
Lane 1: JUP transfected lysate(81.7 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to JUP on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged JUP is approximately 1ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to JUP on A-431 cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (107.69 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant JUP.
Immunogen
JUP (AAH11865.1, 1 a.a. ~ 745 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MEVMNLMEQPIKVTEWQQTYTYDSGIHSGANTCVPSVSSKGIMEEDEACGRQYTLKKTTTYTQGVPPSQGDLEYQMSTTARAKRVREAMCPGVSGEDSSLLLATQVEGQATNLQRLAEPSQLLKSAIVHLINYQDDAELATRALPELTKLLNDEDPVVVTKAAMIVNQLSKKEASRRALMGSPQLVAAVVRTMQNTSDLDTARCTTSILHNLSHHREGLLAIFKSGGIPALVRMLSSPVESVLFYAITTLHNLLLYQEGAKMAVRLADGLQKMVPLLNKNNPKFLAITTDCLQLLAYGNQESKLIILANGGPQALVQIMRNYSYEKLLWTTSRVLKVLSVCPSNKPAIVEAGGMQALGKHLTSNSPRLVQNCLWTLRNLSDVATKQEGLESVLKILVNQLSVDDVNVLTCATGTLSNLTCNNSKNKTLVTQNSGVEALIHAILRAGDKDDITEPAVCALRHLTSRHPEAEMAQNSVRLNYGIPAIVKLLNQPNQWPLVKATIGLIRNLALCPANHAPLQEAAVIPRLVQLLVKAHQDAQRHVAAGTQQPYTDGVRMEEIVEGCTGALHILARDPMNRMEIFRLNTIPLFVQPLYSSVENIQRVAAGALCELAQDKEAADAIDAEGASAPLMELLHSRNEGTATYAAAVLFRISEDKNPDYRKRVSVELTNSLFKHDPAAWEAAQSMIPINEPYGDDLDATYRPMYSSDVPLDPLEMHMDMDGDYPIDTYSDGLRPPYPTADHMLA
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (107.69 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
JUP monoclonal antibody (M01), clone 2G9 Western Blot analysis of JUP expression in A-431 ( Cat # L015V1 ).Western Blot (Transfected lysate)
Western Blot analysis of JUP expression in transfected 293T cell line by JUP monoclonal antibody (M01), clone 2G9.
Lane 1: JUP transfected lysate(81.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to JUP on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged JUP is approximately 1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to JUP on A-431 cell. [antibody concentration 10 ug/ml] -
Gene Info — JUP
Entrez GeneID
3728GeneBank Accession#
BC011865Protein Accession#
AAH11865.1Gene Name
JUP
Gene Alias
ARVD12, CTNNG, DP3, DPIII, PDGB, PKGB
Gene Description
junction plakoglobin
Gene Ontology
HyperlinkGene Summary
This gene encodes a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. Mutation in this gene has been associated with Naxos disease. Alternative splicing occurs in this gene; however, not all transcripts have been fully described. [provided by RefSeq
Other Designations
OTTHUMP00000164735|catenin (cadherin-associated protein), gamma (80kD)|catenin (cadherin-associated protein), gamma 80kDa|gamma-catenin
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Interactome
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Pathway
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Disease
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Publication Reference
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Bioengineered skin constructs based on mesenchymal stromal cells and acellular dermal matrix exposed to inflammatory microenvironment releasing growth factors involved in skin repair.
Luz Correa-Araujo, Leonardo Prieto-Abello, Adriana Lara-Bertrand, Martha Medina-Solano, Linda Guerrero, Bernardo Camacho, Ingrid Silva-Cote.
Stem Cell Research & Therapy 2023 Oct; 14(1):306.
Application：ICC, Human, hWJ-MSCs.
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Bioengineered skin constructs based on mesenchymal stromal cells and acellular dermal matrix exposed to inflammatory microenvironment releasing growth factors involved in skin repair.
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