ISL1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Western Blot
Lane 1: ISL1 transfected lysate ( 38.7 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
ISL1 transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ISL1 full-length
Host
Human
Theoretical MW (kDa)
38.17
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ISL1 antibody (H00003670-B01) by Western Blots.
Western Blot
Lane 1: ISL1 transfected lysate ( 38.7 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
ISL1 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ISL1
Entrez GeneID
3670GeneBank Accession#
NM_002202Protein Accession#
NP_002193Gene Name
ISL1
Gene Alias
Isl-1
Gene Description
ISL LIM homeobox 1
Omim ID
600366Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq
Other Designations
ISL1 transcription factor, LIM/homeodomain|islet-1
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