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Last updated: 2017/8/20
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ISL1 monoclonal antibody (M01), clone 1A3

  • Catalog # : H00003670-M01
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a partial recombinant ISL1.
  • Immunogen:
  • ISL1 (NP_002193, 63 a.a. ~ 159 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • YCKRDYIRLYGIKCAKCSIGFSKNDFVMRARSKVYHIECFRCVACSRQLIPGDEFALREDGLFCRADHDVVERASLGAGDPLSPLHPARPLQMAAEP
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG2a Kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.

    QC Testing of H00003670-M01
    Western Blot detection against Immunogen (36.41 KDa) .
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • ISL1 monoclonal antibody (M01), clone 1A3. Western Blot analysis of ISL1 expression in Hela S3 NE(Cat # L013V3 ).
  • PDF DownloadProtocol Download
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • ISL1 monoclonal antibody (M01), clone 1A3. Western Blot analysis of ISL1 expression in K-562(Cat # L009V1 ).
  • PDF DownloadProtocol Download
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of ISL1 expression in transfected 293T cell line by ISL1 monoclonal antibody (M01), clone 1A3.

    Lane 1: ISL1 transfected lysate(38.7 KDa).
    Lane 2: Non-transfected lysate.
  • PDF DownloadProtocol Download
  • Sandwich ELISA (Recombinant protein)
  • Detection limit for recombinant GST tagged ISL1 is approximately 30ng/ml as a capture antibody.
  • PDF DownloadProtocol Download
  • ELISA
  • RNAi Knockdown (Antibody validated)
  • RNAi Knockdown (Antibody validated)
  • Western blot analysis of ISL1 over-expressed 293 cell line, cotransfected with ISL1 Validated Chimera RNAi ( Cat # H00003670-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ISL1 monoclonal antibody (M01), clone 1A3 (Cat # H00003670-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
  • PDF DownloadProtocol Download
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • ELISA
  • RNAi Knockdown (Antibody validated)
  • RNAi Knockdown (Antibody validated)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 3670
  • Gene Name:
  • ISL1
  • Gene Alias:
  • Isl-1
  • Gene Description:
  • ISL LIM homeobox 1
  • Gene Summary:
  • This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq
  • Other Designations:
  • ISL1 transcription factor, LIM/homeodomain,islet-1
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